International Publication 2011

1 J. Trop. Life Science vol I. no 2. Jun, 2011. 27-32

Influence of Porang (Amorphophalus muelleri) Cultivation On The Composition of Soil Arthropods In Tropical Agroforestry Areas In East Java, Indonesia

Amin Setyo Leksono1*, Bagyo Yanuwiadi1, Zaenal Kusuma2, Akbar Farid1, Fujianor Maulana3

1Biology Department, Faculty of Mathematic and Sciences,  Brawijaya University,  Indonesia
2Soil Department, Faculty of Agryculture, Brawijaya University,  Indonesia
3Postgraduate Program, Brawijaya University,  Indonesia

Abstract

This research was conducted on July to October 2010 to evaluate the effect of porang cultivation on the abundance, diversity and composition of soil Arthropods. Change in the abundance, diversity and composition of soil Arthropods was observed using pitfall traps in two study sites (Jember and Madiun). In each study site, samplings were performed in porang cultivation land and non-porang area. A total of 15 traps were put in three lines at each location. The differences in the abundance and diversity were analyzed by using multivariate analyze tests. The soil Arthropod compositions in all locations were compared with Bray-Curtis similarity index. The effect of environmental factors on the arthropod compositions was analyzed using multiple regression. The result showed that the effect of porang-cultivation was not consistent in two study sites. In Madiun, the abundance of
soil Arthropods in porang cultivation land was slightly higher than that in non-porang, while the diversity was significantly higer in the former location. In contrast the those in Jember was found in vice versa situation. This study indicate that the effect of porang-cultivation was not subtantial to the  composition of soil Arthropods. Therefore practice non harmful porang cultivation should be promoted as a part of agroforestry sistem in both study sites.

Keywords: Agroforestry, conservation, pitfall traps, Soil Arthropods composition

e-mail: leksono72@yahoo.com

2 J. Trop. Life Science vol I. no 1. Jan, 2011. 5-8

Andrographolide  Ameliorate Rheumatoid Arthritis by Promoting the Development of Regulatory T Cells

Muhaimin Rifa’i*

Biology Department, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia

Abstract

Andrographolide  is  important material  present  in Andrographis paniculata.  This material  can  promote  T  cell  to develop  into  regulatory  T  cell,  CD4+CD25+.  CD4+CD25+ regulatory  T  (Treg)  cells,  a  component  of  the innate  immune   response,  which   play   a  key   role    in  the  maintenance  of  self-tolerance,  have   become  the focus  of  numerous  studies  over  the  last  decade.  These  cells  have  the  potential  to  be  exploited  to treat autoimmune disease. In  this  experiment we  showed  that  the  induction  of  regulatory  cells  in  vivo  has  a  healing effect  on  mice  model  of  rheumatoid  arthritis  (RA).  RA  mice  which  is  intraperitoneally  injected     with
Andrographis  paniculata  shows  the  effect  of  recovery.  CD4+CD25+ regulatory  T  cells  actively  control  the properties  of  other  immune  cells  by  suppressing    their    functional    activity    to    prevent    autoimmunity.  In this  experiment  we   showed   that  active compound  from Andrographis paniculata  namely  andrographolide  can induce  active  regulatory T  cell  that has an efficacy to cure rheumatoid arthritis in mice model.

Keywords: Andrographolide; Andrographis sp; Regulatory T cells; Rheumatoid arthritis

e-mail: rifa123@ub.ac.id

3 J. Trop. Life Science vol I. no 2. Jun, 2011. 1-5

Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain)

Harijati N1*,  Widyarti S1, Azrianingsih R1

1Biology Department, Faculty of Mathematic and Sciences,  Brawijaya University, Indonesia

Abstract

One of  indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower  obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include  Amorphophallus muelleri,  A.variabilis  (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus  (Var Selopuro).  Amorphophallus  diet was given to white rats of Wistar strain that
previously fattened using a mixture of cholesterol and lard, in addition to PARS  (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1).  Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus  (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol)  diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been
able to reduce cholesterol to the end of observation (25 days).

Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac

e-mail: harijati@ub.ac.id

4 J. Trop. Life Science vol I. no 1. Jan, 2011. 1-4

Assessment of bio-activities of the crude extract and components of Withania somnifera leaves by bioinformatics

Widodo1, Didik Priyandoko3, Tetsuro Ishii3, Custer Deocaris2, Renu Wadhwa2*, Sunil C. Kaul2

1.Department of Biology, Faculty of Sciences,  Brawijaya  University, Indonesia
2.National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan
3.Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan

Abstract

Traditional herbal medicines are now increasingly being appreciated with Western models of integrative health sciences and evidence-based approach both in  the basic  research and  clinic scenario. Ashwagandha is  a commonly used plant in Ayurvedic, Indian traditional medicine. Medicinal value of Ashwagandha (WithaniasomniferaDunal) extends from anti-inflammatory, anti-arthritic, anti-rheumatic, rejuvenation and anti-cancer. Based on the belief that  holistic multi-site mechanism  of action offers greater chance  of  success, the traditional  Ayurvedic medicine  practices the use of  whole herb or its  crude extract. It  opposes  with the mainstream of pharmaceutical  industry  that  uses  single  and purified molecules. In the present study, we  used bioinformatics approach to reveal the mechanism of action of (i) crude extract of Ashwagandha leaf extract and its purified components, (ii) Withanone and  (iii) Withaferin A. Whereas p53-p21 was identified as a common signaling pathway for the three kinds of reagents, specific signaling pathways for Withaferin-A and Withanone were identified. Whereas the crude extract and Withanone were selectively toxic to human cancer cells, WithaferinA showed cytotoxicity to the  normal cells too. The study suggested that the crude extract or a combinational formulamay be a superior and safe natural reagent for cancer treatment.

Keywords: Holistic mechanism, traditional medicine, Withania somnifera

e-mail: renu-wadhwa@aist.go.jp

5 Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Potency of Inhibin B As Male Hormonal Contraception Candidates Based on Sperm Viability and LH Serum Level of Rattus norvegicus

Muhammad Hilman F. Amin l, Aulanni’am 2, Agung P.W.Mahendra3, Muslim Akmal4

(1,3 )Department of Biology, Faculty of Sciences, Brawijaya University, Malang
(2 )Department of Chemistry, Faculty of Sciences, Brawijaya University, Malang
(4 )Faculty of Veterinary Medicine, University of Syiah Kuala, Aceh

Abstract

Large population, in addition to being capital for country’s development is also contrary be problem and burden for the country. One of effort to control increasing population is contraception. Men should also have same burden in family planning, so it is extremely urgent to develop new, safe, effective, and reversible male contraceptive methods. Among all male contraceptive methods, hormonal approach is the closest to clinical application. One of requirements of an ideal male contraceptive is not affected masculinity and libido, which regulated by testosterone-LH. Inhibin B is hormone which controls FSH secretion, whereas FSH is central hormone of mammalian reproduction,necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life. The purposes of this study were to determine role of inhibin B from primary culture of testicular to sperm viability and serum LH levels of rats (Rattus norvegicus) Wistar strain. This study used four groups of treatment, ie K0, K1, K2 and K3 are each injected with inhibin B in PBS with a dose of 0, 25, 50, and 100pgper 200 g body weight (BW) rats. Sperm viability was tested by eosin-nigrosin dye and was examined under light microscopy in 400x magnification, and measurement of serum LH levels through ELISA method. The results showed that administration of inhibin B can reduce viability of spermatozoa significantly, but did not reduce levels of LH in serum. This indicates that inhibin B has potential to be developed as a male hormonal contraceptive.

Keywords: contraception, inhibin B, LH, sperm viability.

e-mail: muhammadhilman87@gmail.com

6 Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Diversity, Composition, and Community Structure of the Teak Canopy Arthropods in Porang Crop Area Saradan Forest, Madiun, East Java

Syahrudin Agung Permana 1, Amin Setyo Leksono 1, Bagyo Yanuwiadi 1

(1 )Department of Biology, Faculty of Science, University of Brawijaya

Abstract

Increasing of management Porang (Amorphophallus muelleri Blumei (Schott) Engl.) productions were to developes the forest peoples with agroforestry system on the base of forest.areas. This research aimed to analyse and to compare diversity, composition, and community structure of Canopy Arthropod in the different location (Porang Crop area and Non-Porang Crop Area of agroforestry forest) and both of correlation with its abiotic factors. Sampling methods performed by adopting yellow and blue colored water pan trap hanged on the trees (5-15m). The samples were identified and analysed in the Laboratorium. Bray Curtis Index, Diversity (Shannon-Wienner), and number of important values index measured to compare of Community structure between two areas. A total of 283 specimens belong to 9 orders and 19 families of the Canopy Arthropods were collected in Porang Crop area and 560 specimens belong to 10 orders and 22 families of the Canopy Arthropods in Non-Porang Crop area. The yellow traps had better trapping efficiency than the blue traps in abundance. The result showed the significant difference in abundance among two areas (F-test, P < 0.05). The value of both communities was 0.788 were measured in this research, indicated that the difference of Arthropods composition had a similarity. Community Structure was dominated by Simuliidae in the both of areas with number of important species index 60.48 in the porang crop area and 73.03 in the non-porang crop area. There was significant light intensity decerasing and this fact affected on the canopy Arthropods abundances. It increases with the negative correlation of -0.582. The significant air moisture decreasing was affected on The Canopy Arthropods diversity with the negative correlation of -0.515.

Keywords: Community structure, composition, diversity, porang, canopy arthropods

e-mail: syahrudin.agung@gmail.com

7 Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Role of Wild Plant as Alternative Habitat for Natural Enemies in Agricultural Land

Wiwin Maisyaroh 1,Bagyo Yanuwiadi 2, Amin Setyo L. 2, Zulfaidah P.Gama 2

(1)Graduate School of Mathematics and Science Brawijaya University, Malang, Indonesia
(2)Department of Biology, Brawijaya University, Malang, Indonesia

Abstract

Use of chemicals in the agricultural industry is known to provide a considerable impact is also alarming for the health and sustainability of ecosystems. The use of improper chemicals in controlling pests such as pesticides can give side effects such as target pests becoming resistant to pesticides and the occurrence of pests resurjensi. Effort in maximizing the performance of natural enemies in agricultural land one of them is by creating habitat favored by natural enemies, habitat management is done to increase the number of natural enemy populations and maximize its role in controlling pests. The purpose of this study is to determine interest in natural enemies of insect pests of rice to some wild plants is: Mimosa pudica, Vernonia cinera, Marsilea crenata, and Pistia startiotes in rice plants. This research was conducted in March-April 2010 in Malang Sawojajar farmland. This research was done by placing a patch of weeds in rice fields located at the corner of the block, hereinafter referred to as refugia, attraction of insects were observed ranging from refugia blocks and each block distance of 2 meters away from the refugia. Observation method used is the method of “visual control” developed Freie and Manhart (1992). The results showed that the insects that visit refugia block consists of 9 orders namely: Coleoptera, Odonata, Aaraneae, Hymenoptera, Diptera, Homoptera, Orthoptera, Lepidoptera, and Hemiptera. Insect visitors refugia block consisting of 24 families and is divided into 3 roles of predators, parasites, and pests. Of the 24 families of the 15 family is the natural enemy insects and 9 family is an insect pest. In the first plot of insect natural enemies are most of the family Formicidae, whereas on the plot the second, third and fourth largest insect natural enemies is of the family Coccinellidae. The second largest number in the plot first, second, and fourth is from the family Aeshnidae, while the number of insects at least almost in each plot is the insect family Lycosidae.

Keywords: Refugia, Natural Enemies, Visual Control.

e-mail: may_nis@yahoo.co.id

8 Proceedings of The International Conference on Basic Science 2011, Brawijaya University

The Effect of Refugia Blog to Spatial and Temporal Distribution of Natural Enemies in Rice Field

Asyik Nur Allifah AF l, Bagyo Yanuwiadi2, Zulfaidah P. Gama2,Amin S. Leksono2

(1 )Magister Program, Faculty of Sciences, Brawijaya University, Malang, Indonesia
(2 )Department of Biology, Faculty of Sciences, Brawijaya University, Malang, Indonesia

Abstract

The aims of this research were 1) to know the effect of refugia blog to the amount and type of natural enemy visitors of refugia blog, 2) to know the effect of refugia blog to the pattern of spatial and temporal distribution of natural enemies. The study has been conducted on the farmland in Sekarpuro Pakis, Malang regency at May to October 2010. Refugia collections consist of a group of four plant species (Eupatorium odoratum, Mimosa pudica, Brachiaria mutica, Panicum repens). Refugia blog sized 1×1 m2. Observations were done as many as 4 periods: the first period were done at 07:00 to 8:00 pm, second period at 9:00 to 10:00 pm. Third period at 12:00 to 13:00 pm and the fourth period at 3:00 pm to 16:00 pm. Observation consist of 4 observation plots with spatial 2 m. Length of observation 15 minutes. Observation method used is modification of the “visual control”. Analysis was done by factorial design ANOVA. The result of this research showed that natural enemies with the mostly arrived was Coccinellidae family (71.47 ± 5.09), while the fewest was Tettigonidae family (3.53 ± 0.84). Effect of refugia blog showed significantly affect (P<0,05) to temporal distribution and not significantly affect (P>0,05) to spatial distribution of Arthropod in rice field.

Keywords: refugia blog , spatial and temporal distribution, natural enemies

e-mail: yanuwiadi@ub.ac.id

9

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Woody Plant Diversity and Structure of Coffee Agroforests in the Recharge Area of Some Springs in Ngantang Subs District, Malang Regency, East Java

Titut Yulistyarini1, Zaenal Kusuma2, Soemarno 2, Endang Arisoesilaningsih 3

(1)Purwodadi Botanical Garden, Indonesian Institute of Sciences (LIPI), Pasuruan Regency, East Java
(2)Soil Department, Faculty of Agricultures, Brawijaya University, Malang, East Java, Indonesia
(3)Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang,East Java, Indonesia

Abstract

Ngantang have potential as a regional distribution of springs, there are about 71 springs distribute in a group. More than half of them discharged in a small debit, less than 5 l/sec. Krisik, Complang and Pusung pegat springs discharged about 1 l/sec. These springs have decreasing water debit, the last two springs even produce no more discharge. The recharge areas of third springs were dominated by coffee agroforestry land cover. This study to compares woody plant diversity and structure among coffee agroforests in the recharge area of each spring. Woody vegetation was sample in plots 100x 20 m2 to class of tree (dbh > 30 cm) and in sub plots 40×5 m2 to class of small tree (dbh < 30 cm). Woody species diversity of Krisik was lower than diversity of Complang and Pusung pegat. Tree species of Krisik and Pusung pegat had a highest similarity index.

Keywords: Diversity, cofee-agroforest, woody vegetation, spring.

10

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Single Nucleotide Polymorphism Identification of Porang in East Java

Estri Laras Arumingtyas

Department of Biology, Faculty of Science, University of Brawijaya, Malang

Abstract

Porang (Amorphophallus muelleri Blume) belong to the genus of Amorphophallus which is is a large genus of some 170 tropical and subtropical tuberous herbaceous plants from the Arum family  (Araceae). Twenty seven species of Amorphophallus are found in Indonesia. Amorphophallus species are valued for its tuber glucomanan among the species found in Indonesia; A.muelleri Blume is a species with the highest content of glucomannan. Many variants of this species were grown in different places at East Java. Based on morphological characters it is difficult to differentiate between them. To identify whether they were similar variety, a Single Nucleotide Polymorphism (SNP) of non-coding sequence, intergenic region in the chloroplast DNA, trnL-F, was studied using PCR technique. Primers used were trnL-F r 5′-CGA AA T CGG TAG ACG CTA CG-3′ and trnL-F f 5′- ATT TGA ACT GGT GAC ACG AG-3′ The program used was pre heating at 96°C for 2 minutes, followed by 36 cycles of denaturation at 96°C for 30 seconds, primer annealing at 56°Cfor 30 seconds and elongation at 72°Cfor 120 seconds, and the last extention at 72°C for 7 minutes. The PCR resulted in a single band of about 500 bp. Sequencing result show that all the 14 samples from 5 places at East Java had exactly similar sequence that suggested that all sample were similar varieties. Alignment with trnL of Amorphophallus muelleri Blume from NCBI, show that the samples were similar to the species Amorphophallus muelleri Blume.

Keywords: Amorphophallus muelleri Blume, polymorphism, SNP, glucomannan.

e-mail:  laras@ub.ac.id

11

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Function of hINSR Mutant Againts Tyrosine Kinase Precede Abnormally on Onset Diabetes Mellitus: In Silico Study

Fatchiyah 1,2, NMA. Putri 3, D. W. Soeamadji4

(1,3 )Dept of Biology, Faculty of Science, Brawijaya University, Malang
(2 )Central Lab. of Life Sciences (LSIH), Brawijaya University, Malang
(4 )Medical Faculty, Brawijaya University, Malang

Abstract

The pathogenesis of NIDDM has been studied in various ethnic groups. It appears that insulin resistance can precede the clinical onset of NIDDM. Mutation of the human insulin receptor gene have been identified in patients with severe insulin resistence, and studies of these naturally occuring mutants may provide important insights into the relationship between structure and function of receptor (hINSR) of Indonesian diabetes mellitus patients. The bloods were collected from normal and DM patients from some public clinics and Saiful Anwar Hospital, Malang. DNA and RNA were isolated from blood, and then sequenced by ABIPrims Sequencer. To find out the genomic hINSR, DNA sequences were analyzed and characterized by in silicoanalysis, such as alignment by BioEdit & BLAST program from NCBI, and superimposed by Strap JAVA program, 2D- and 3D-structure analysis Swiss Model program. To examine the cytoplasm pathway tyrosine kinase, using docking hINSR-Tyrosine Kinase domain & IRS-1 (PTB domain) analyzed by Hex 5.1. We found specific of DM protein from 2D-protein profile and some type mutation of hINSR and can change the INSR 3D-protein structure and the 3D ligand structure of hINSR and insulin completely changed on DM patient. According to our result, we suggested that the hINSR protein mutation of DM patient precede abnormally hINSR function against tyrosine kinase and perhaps correlated with genetic syndrome of insulin resistance. The change function is presumed to inhibit the interaction between hINSR and IRS, makes transduction signal disturbance in the process of absorption of glucose leads to insulin resistance of diabetes mellitus.

Keywords: insulin receptor, diabetes mellitus, genetic syndrome, in sillico, tyrinose kinase.

e-mail: fatchiya@gmail.com

12 Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Immune System Impairment in CD122-Deficient Mice is Caused by Abnormality of Activated T Cells

Muhaimin Rifa’i

Biology Department, Faculty of Sciences, Brawijaya University. Malang Indonesia

Abstract

Regulation of immune system is a pivotal to prevent immune attacks against self-components. Mice deficient in the IL-2/IL-15 receptor ft chain, CD122 are model animals of such immune attacks and characteristically have a high number of abnormally activated T cells. Here we show that CD122-deficient mice dominated by the development of abnormal T cells. In this study we found that CD4 and CD8 T cells express high level of CD69 and CD44 molecules. Furthermore, the expression of CD62L molecule becomes low and the number of granulocytes cell increased dramatically. This result indicating that normal cells become dangerously activated T cells in the absence of CD122 endogenous molecule.

Key words: regulatory T cells • CD8+ T cells • CD122 • activated T cells

e-mail: rifa123@ub.ac.id

13

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Exogenous IL-2 Promote T Cells Proliferation Under Anti -CD3/CD28 Combination

Muhaimin Rifa’i

Biology Department, Faculty of Sciences, Brawijaya University.

Abstract

Strategy of both activation and proliferation of the cell interest in vitro plays a very important role for clinical purpose. In this experiment we observed that exogenous IL-2 is required to maintain the survival of activated T cell in vitro. Here we also demonstrated that T cell expansion derived from lymph node activated with anti-CD3/anti-CD28 combination was greatly enhanced in the presence of exogenous IL-2. In the absence of exogenous IL-2 the cell become more susceptible to apoptosis and or necrosis. In the stimulation of anti-CD3/anti-CD28 combination, CD8+ T cells were preferentially stimulated. T-cell activation which plays a central role in the regulation of immune responses, involves multiple intracellular signaling events originating from the cell surface TCR/CD3 complex. Cross-linking of the TCR/CD3 complex by anti-CD3 antibody induces T cell activation, leading to the production of cytokines. We found evidence that stimulated CD8 T cells particularly CD8 CD122 subpopulation express high level of IFNy, while CD4 particularly CD4+CD25-express high level of IL-2. Interestingly CD8+ T cells when stimulated with combination of anti-CD3/anti-CD28 could mimic the cell surfice molecules to double positive CD8+CD4LowT cells.

Keywords : PLN, anti-CD3/anti-CD28, IL-2.

e-mail: rifa123@ub.ac.id

14

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Monoclonal Antibodies to Bovine Zonna Pellucida 3 (Mab-bZP3) for Woman Immunocontraceptive Vaccine

Dedy Kurniawan 1, Sutiman B Sumitro 1, Aulanni’am 2

(1)Department of Biology, Faculty of Mathematics and Sciences, Brawijaya University, Indonesia
(2)Department of Chemistry, Faculty of Mathematics and Sciences, Brawijaya University

Abstract

Immunocontraception has received increased interest in last decades as a potential way of controlling the rapid growth of Indonesian population. Development of vaccine based on zona pellucida antigens represents a promising approach to potential contraception.. Recent advances in monoclonal antibody technology are enabling the development of new methods for producing specifif antibody for immunocontraception. Seven research stages were conducted to construct monoclonal antibodies to Bovine Zonna Pellucida 3 (Mab-bZP3). Stage 1, collection Bovine Zonna Pellucida 3 (bZP3), stage II, Immunisized mouse Balb/c by Bovine Zonna Pellucida 3 (bZP3), stage III, Collecting spleen cells from a mouse that has been immunized with the desired antigen, stage IV Fussion spleen cells with myeloma cells, stage V, Hybridoma screening , stage VI, single cell cloning and Monoclonal antibody production and characterization. The Result showed that Mab-bZP3 were produced both hybridoma cell and ascites fluid positively and more specific reacted with antigen Bovine Zonna Pellucida 3 (bZP3) by immunoblotting technique. Mab-bZP3 should prove useful for candidate immunocontraceptive vaccine.

Keywords: Bovine Zonna Pellucida 3 (bZP3), immunocontraception, monoclonal antibody.

e-mail: rudededy@gmail.com

15

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Characterization of Allergen Proteins and Alergenicity of Porang tubers (Amorphophallus muelleri Blume.) by using IgE-Immunoblotting

Sofy Permana, Sri Widyarti, Luccy Mutia Lingga Dewi, Indrian Rizka Amalia

Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia

Abstract

The aims of this research were characterizing the allergen proteins and comparing the allergenicity between the raw and steamed-porang tubers by using IgE-Immunoblotting. Three variants of Porang: Klangon-Madiun, Sumber Baru-Jember and Sumber Bendo-Madiun were observed. Protein preparation was done according to Li et al. (2003) method, separated using SDS-PAGE electrophoresis, stained by silver and blotted with semi-dry transfer. Mouse serum with highest titer of IgE from ELISA was used as primary antibody, and Goat anti-Rat IgE Biotin Conjugated was used as secondary antibody. The density of protein bands were analyzed by Chemidoc Gel Imaging and Quantity One 4.6.8 software. The result of IgE-Immunoblotting of those three raw and steamed-porang tuber variants showed that the allergen protein bands profile were similar in each variant, with the appearance of molecular weight 26, 32 and 43 kDa. The density of a 26 kDa allergen was the highest protein in raw-porang. In the other hand, a 32 kDa allergen was the highest protein density in steamed-porang from Sumber Bendo, and a 43 and a 32 kDa allergens were in steamed-porang from Sumber Baru. The density of allergen in steamed-porang from of Klangon was in 26 kDa, but it was lower than that of raw-porang one. These suggest that both raw and steamed-porang had potency to induce allergy.

Keywords: Allergen, protein Porang, ELISA, IgE-Immunoblotting.

e-mail: sofybraw@yahoo.com

16

Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Growth Differentiation Factor-9 Gene Expression of Mice Oocytes in Vitro

Sri Rahayu 1, Manami Nishio 2, Yumi Hoshino 2, Eimei Sato 2

(1 )Departement of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia
(2 )Graduate School of Agricultural Sciences, Laboratory of Animal Reproduction , Tohoku University, Japan

Abstract

Growth differentiation factor-9 (GDF-9) is a growth factor secreted by oocytes in growing ovarian follicles. In mice, the use of ‘knock-out’ models has shown that GDF9 is essential for normal follicular development with BMP15 regulating the fertilization potential of oocytes. The aim of this research was to find out the influenced volume of IVM oocyte media to GDF-9 gene expression. Oocytes were collected by puncturing the surface of the mice ovaries with sterile needles 27G. Furthermore oocytes were matured within Hx medium during 22 hours at 38.5°C temperature in the incubator with 5% CO2 to achive metaphase II (MII) oocytes. Oocytes were cultured in 3 variations media droplet volume of 50 ul / droplet, 100 ul / droplet and 200 ul / droplet. After RT-PCR and agarose gel electrophoresis, relative mRNA abundance of GDF-9 was analyze in each group of oocytes. The GDF-9 gene expressions of oocytes were obtained when oocytes were cultured in 200 ul / droplet media.

Keywords: GDF-9 gene, Metaphase II (MII), mice oocyte.

17 Proceedings of The International Conference on Basic Science 2011, Brawijaya University

Lactobacillus Plantarum S1.30 Isolated from Dadih has Antagonistic Properties and Harbours Gene Encoding the Production of Bacteriocin

Yoga Dwi Jatmiko 1, Miguel De Barros Lopes 2, Mary Barton 2

1)Laboratory of Microbiology, Department of Biology, Faculty of Sciences, University of Brawijaya, Malang, Indonesia
2)School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, Australia

Abstract

Dadih is considered an important traditional fermented milk product with potential health benefits to local Indonesian communities. In addition, dadih lactic acid bacteria have antimicrobial activity believed to be important in increasing the safety and extending the shelf-life of the fermentation products. In this study, Lb. plantarum S1.30 isolated from dadih was assayed for its antagonistic activity and the presence of gene encoding bacteriocin production. Antibacterial activity of Lb. plantarum S1.30 against multidrug-resistant Gram negative pathogens (Salmonella spp. and Escherichia coli) and Gram positive pathogens was tested in vitro using well diffusion agar assay. The presence of bacteriocin activity was tested both conventional method and PCR-based method using specific primers targeting a bacteriocin regulation gene, plantaricin A. Furthermore, the antimicrobial production kinetic of Lb. plantarum S1.30 was also conducted against Listeria monocytogenes and Lactobacillus sakei ATCC 15521 in a liquid medium. The antimicrobial substances produced by Lb. plantarum S1.30 inhibited all the indicator bacterial pathogens, with the Gram positive pathogens were more sensitive than Gram negative pathogens. The bacteriocin detection was failure to be demonstrated using a conventional method. While, the PCR amplification resulted a-450 bp-DNA fragment, which indicated that plantaricin A was present in Lb. plantarum S1.30. The results obtained from kinetic studies of antimicrobial production showed that the organic acids produced by Lb. plantarum S1.30 played an essential role in inhibiting the growth of target bacteria. These result suggested that bacteriocin produced by Lb. plantarum S1.30, called plantaricin, could be regulated by a quorum sensing mechanism.

Keywords: Lactobacillus plantarum S1.30, antimicrobial activity, bacteriocin, dadih.

e-mail: jatmiko_yd@ub .ac.id

18 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Predation of Introduced Cichlids Threaten Native Glassgobies Living in Ranu Grati

A.R.K. Nisa’, E. Arisoesilaningsih, A.S. Leksono, M. Imam

Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang
chabio19@yahoo.com, e-arisoe@ub.ac.id, leksono72@yahoo.com, imam@ub.ac.id

Abstract

The aims of this research were to identify prey composition of two introduced cichlids and compare their predation to local glassgobies in Ranu Grati, Pasuruan, East Java. Flowerhorn and jaguar fish as introduced species were captured using 2 mm fishing net, then their gut was dissected. Preys in the gut were identified, counted and finally weighted their biomass. Data analysis was performed descriptively using Microsoft Excel. Prey composition of flowerhorn diet was dominated by Gobiopterus brachypterus and G. chuno or glassgobies added Poecilia reticulata and Lymnaea javanica. Whereas, prey of jaguar fish was just glassgobies. The result showed that comparasion bundance of prey in each gut flowerhorn and jaguar fish were range between 0-5 glassgobies and 1.1-6.5 glassgobies. While comparasion biomass of prey were range between 0.4-37.2 mg.ind-1 of flowerhorn and 13.7-107.3 mg.ind-1 of jaguar fish. Jaguar fish captured twice more glassgobies biomass than flowerhorn. Its showed that jaguar fish as introduced spesies more aggressive than flowerhorn.

 

Key words: flowerhorn, glassgobies, jaguar fish, predation.

19 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Abundance of Soil Arthropods on Porang (Amorphophalus muelleri) and non-porang Cultivation Tropical Agroforestry Area in Kecamatan Tanggul Kabupaten Jember city, East Java, Indonesia

Akbar Farid1, Amin Setyo Leksono1, Bagyo Yanuwiadi1, Zaenal Kusuma2, Fatchur Rohman3

1 Biology Department, Faculty of Mathematic and Sciences, Brawijaya University, Malang, Indonesia
2 Soil Department, Faculty of Agriculture, Brawijaya University, Malang, Indonesia
3 Biology Department, Faculty of Mathematic and Sciences, Malang University, Malang, Indonesia

Abstract

Soil Arthropods have important role in environmental and high diversity. The aim of this research to explore the comparison of abundance of soil arthropods in porang and non-porang cultivation in kecamatan Tanggul, Jember city. The samples were taken every four days for four times in October (rainy season) using pitfall trap method with three transect in each location. The result showed that Porang and non-porang cultivation was not different significant the abundance of soil arthropods. The soil arthropods in Porang cultivation that have most highly abundance than soil arthropods other are Formicidae, Collembola, Lycosidae, Staphylinidae, and Grylidae family. And the soil arthropods in non-porang cultivation are Formicidae, Collembola, Grylidae, Staphylinidae and Lycosidae.

 

Keywords: Abundance, pifall trap, Porang-cultivation, Soil arthropods.

20 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Genetic Diversity Lawar’s Worm Perinereis cultrifera Grube (1840) In Kei Kecil Islands, Southeast Maluku District

Desy Wulan Ariani1), Widodo2), and Luchman Hakim2)

1) Postgraduate Program Science and Technology Faculty of University of Brawijaya
ecy_cempluk@yahoo.co.id
2) Biology Department,  Faculty of Sciences , University of Brawijaya, Malang

Abstract

Lawar’s Worm (Perinereis cultrifera) is one of biological resource in the archipelagic marine of Kei Kecil islands, Southeast Maluku District especially at Wearlilir and Langgur marine, these organisms can be found in the clean and clear marine, which is hard substrate covered with algae, corals, and coral reefs that have not been damaged [1].Genetic diversity is considered to be important because it is an important key for a species to survive until the next generation and as a conservation effort. One of the molecular analyze that often used is the RAPD method (Random Amplified Polymorphic DNA), using OPA primers.The results showed that RAPD analysis using 10 OPA’s primers (OPA 1, OPA 4, OPA 5, OPA 6, OPA 7, OPA 8, OPA 9, OPA 10, OPA 16, OPA 17) screening on the Lawar ‘s worm population at Wearlilir and Langgur very various and able to produce ± 59,69% polymorphic bands. The level of genetic similarity of each population can be illustrated by a genetic distance of individuals members of the population. Similarity index between individuals in populations Langgur range of 0.531-0,859, while the index of similarity between individuals on the populations Wearlilir range of 0.554-0,729. And then Similarity index between two populations range of 0,533-0,87.

Key words: genetic diversity, Kei Islands, Perinereis cultrifera

21 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Wood Yield and Energy Characteristics Produced by Several Local Fast Growing Trees Grown in the Purwodadi Botanical Garden

Hellen Aulia Putri1, Endang Arisoesilaningsih1, Lailatin Nuriyah2 and Soejono3

1Biology Department, Mathematics and Natural Sciences Faculty, Brawijaya University, Malang, Indonesia
henyend@gmail.com, e-arisoe@ub.ac.id
2Physics Department, Mathematics and Natural Sciences Faculty, Brawijaya University, Malang, Indonesia
lailatin@ub.ac.id
3UPT Kebun Raya Purwodadi, LIPI, Indonesia
soejono@lipi.go.id

Abstract

The  aims of this study were to determine wood yield and energycharacteristic of several local species grown in the Purwodadi Botanical Gardens (KRP) used as firewood. Quality of firewood was analysis based on the wood yield,energy capacity and longevity of ember. Data were analyzed descriptively using Microsoft Excel 2007. The results showed that trees of Cordia bantamensis, Dysoxylum amooroides, Parkia timoriana, Peltophorum pterocarpa and 25 others species producedhigher wood yield. Their annual wood yieldreached ≥23 kg C.year-1. Wood of Firmiana sumbawanensis, Gluta renghas, Syzygium javanicum, Calophyllum soulattri, Heritiera littoralis, Michelia alba, Garcinia balica and Cathormion umbellatum generated high calorific value amount and reached more than 4.0 kkal.g-1. Whereas Cananga odorata, Kleinhovia hospita, Antidesma bunius, H.littoralis and Phyllanthus acidus were recommended as firewood based on their high longevity of ember more than 10 minutes.g-1. Its seemed that longevity of ember was positively influenced by annual woodyield. Overall, we proposed to plant Cathormion umbellatum and Cananga odorata for producing high wood yield, high calorific value and long ember. While for producing high calorific and long ember is better to plant H. littoralisfor reforestation.

 

Keywords: annual wood yield, energy capacity, firewood, longevity of ember

22 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Community Structure of Soil Arthropods in Tambakrejo Beach, Blitar as a Quality Indicator of Coastal Tourism Ecosystem

Hilmiyyah Yulianti1, Luchman Hakim2 and Amin Setyo Leksono3

1,2,3 Biology Department, Faculty of Mathematics and Science, Brawijaya University, Malang, Indonesia
1hilmiyyah.yulianti@gmail.com2lufehakim@yahoo.com3 leksono72@yahoo.com

Abstract

Maintaining the quality of ecosystems is necessary for the sustainability of coastal tourism industry. Soil arthropods is one of the biological indicator to evaluate quality of ecosystems. The aim of this study were to compare the abundance, diversity, composition and community structure of soil arthropods between two sites with different levels of tourist activity (high and low) and to analyze the relationship between soil arthropods related to environmental factors. Pittfall traps were used to collect soil arthropods. Soil arthropods which collected from site with high tourist activity was 5062 specimens (11 orders, consisting of 17 families) and from low tourist activity was 1071 specimens (12 orders, consisting of 18 families). The diversity of soil arthropods in site with low level of tourist activity was greater (H ‘= 1,58) compared to site with high level of tourist activity (H’ = 0,80). Composition of arthropods in two sites did not differ (Morisita similarity index was 0,97). Community structure of soil arthropods in two sites was dominated by family Formicidae. Declining of soil temperature, air temperature and light intensity had impact on increasing soil arthropods abundance significantly, but soil pH had no significant effect. Based on F-test result, it was found that the difference level of tourist activity lead to significant changes on the abundance of soil arthropods, but did not cause significant changes on diversity level at the two sites.

 

Keywords: Arthropods, indicators, community structure, coastal tourism.

23 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Contribution Canopy Arthropod to Increase Stability Ecosystems in Garden Based Sengon Laut (Paraserianthes falcataria L.) of Porang (Amorphophallus muelleri Blumei). (Schott) Crop Area, Jember

Ivone Wulandari Budiharto1, Amin Setyo Leksono1, Zaenal Kusuma2, Bagyo Yanuwiadi1

1 Biology Departement, Faculty of Mathematic and Science, Brawijaya University, Indonesia
2 Soil Departement, Faculty of Agriculture, Brawijaya University, Indonesia.

Post Graduate of Mathematic and Science, Brawijaya University, Indonesia
Alyanda_maniez@yahoo.com

Abstract

The development of agroforestry based of Sengon Laut with Porang crop area has successfully to increase people economic level. This research aimed to analyze and compare diversity, composition, structure community of canopy Arthropod at two different location (Sengon Laut of Porang crop area and non porang crop area) which was attracted to blue and yellow colored water pan trap, vertical variation diversity, abundance, and structure community of canopy Arthropod, to know the relation between community of Arthropod canopy with abiotic factor at local agroforestry and to recommend good cultivating management. Samples of canopy Arthropod was taken at two different location by blue and yellow colored water pan trap, which was hang on bottom layer (1-1,5 m) and upper layer (8-10 m). Abiotic factors (Temperature and light intensity) were measured at each location. Non crop vegetation was analyzed by line intercept method and tree vegetation was analyzed by complete count method. Data of structure community comparison each location was analyzed by important value and diversity (Index Shannon-Whienner). The degree of same level composition between two location was analyzed by Index Bray-Curtis and variation vertical analyzed by anova. Canopy Arthropod composition and relation with abiotic factors was analyzed by Pearson Correlation with MINITAB program. The value abundance of canopy Arthropod in Porang crop area was (329 individu), fewer than canopy Arthropod in non Porang crop area which was (829 individu). The result showed that yellow water pan trap had better efficiency on upper layer at non Porang crop area than porang crop area. The value Diversity canopy Arthropod index from non Porang crop area was (2.56) more than Porang crop area which was (2.05). The abundance of canopy Arthropod among two locations, color, and hight were significant different with PRecommendation to the farmers in order to make good cultivation management non crop vegetation is not cleaned and not used pesticide utilization.

Keywords: abiotic factor, agroforestry system, canopy arthtropod, diversity, structure community, vertical variation.

24 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Composition and Diversity of Arthropods in Porang Fields in Madiun

 

Siti Maimunah1, Vivi Apriliya 2, Amin Setyo L.3 and Fatchur Rohman4

1, 2, 3 Biology Departement, Faculty of Science, Brawijaya University, Malang, Indonesia
4 Biology Departement, Faculty of Science, State University of Malang, Indonesia
siti.mai354@gmail.com, aprilia_surya@yahoo.com, leksono72@yahoo.com

Abstract

Arthropods can be used as indicator of healthy forest in accordance with its abundance. The aim of its research were to study the structure of vegetation and its correlation to the abundance of Arthropods in four different areas.  Arthropods were collected from each field using fly net. The result was different in the type of vegetation and abundance of Arthropods. In location under shade of Dalbergia latifolia canopy, we found that the diversity of both plant vegetation and Arthropods were greater than in area without canopy. The diversity index of plant vegetation was 0.7 in area under shade of canopy and 0.5 in area without canopy. The diversity index of Arthropods was 1.25 in the area under canopy and 0.9 in area without canopy. The diversity of Arthropods in porang field was 1,265 and in non porang field 1,150. The diversity of plants and Arthropods were low.  The value of Bray-Curtis Index was 0.398, it showed that the composition of Arthropods in two areas were different.

 

Keywords: Arthropods, canopy, diversity, porang

25 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Increasing Human Activities and Impact to Eutrophication of Volcanic Lake Ranu Grati

Shelie Puspitasari , Endang Arisoesilaningsih, Bagyo Yanuwiadi, Muhammad Imam

Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia
sheliepuspitasari@yahoo.co.id, e-arisoe@ub.ac.id, yanuwiadi60@yahoo.co.id, imam@ub.ac.id

Abstract

The aims of this research were to study dynamic of local communities activities related to the lake and their contribution to influence eutrophication. Human activities during 1985-2010 were studied by semi-structural interview. The fresh water quality were determined by measuring temperature, brightness, conductivity, pH and dissolved oxygen (DO) in 3.0 m of depth. Qualitative data were analyzed using Microsoft Excel, while the quantitative data were compared to previous data and 2nd class standard of water quality. The results showed that the utilization of Ranu Grati since 1985-2010 has increased from three to eight activities. Local community previously used lake as toillets, sand mining, rice fields, then installed floating net cage of some exotic fish, tourism, garbage discharge, farms and finally they developed floating cages of local glassgobies. Most people >60% reported decreasing fish harvest as declining water quality, but less awareness were observed. Based on the brightness of water reached 99-140 cm and <200 cm, Ranu Grati was classified as eutrophic lake. In addition, the DO rate varied 2.5-3.7 mg.l-1 and some times it was lower than the standard (4 mg.l-1). This decreasing DO was an indicator of lake eutrophication, it was seemly caused by loading anthropogenic organic waste.

 

Keyword : abiotic factors, DO, eutrophic, organic waste, quality standard

26 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Aqueous Extract Datura metel Flowers Induce T Lymphocyte Activity and Alteration Of Bronchioles Histopathology In Murine Balb/c Model Asthma

Dewi S1., Muhaimin Rifa’i1, Aulanni’am2

(1) Biology Departement,  Faculty of Sciences , University of Brawijaya, Malang
debonkbonk@yahoo.com
(2) Chemistry Departement,  Faculty of Sciences , University of Brawijaya, Malang

Abstract

Asthma occur as a result of allergen exposure into airway. It’s induce immune response, marked by proliferation and differentiation of naive cells, include T cells, and alteration histopathology of bronchiolus in mice BALB/c model asthma. Datura metel flowers believed to cure asthma. The aims of this study were to know T cell lymphocyte activity, determine efective dosage of D.metel to cure asthma and alterations of bronchiolus histopathology post alergen challege and oral administration of D.metel flowers aquoeus extract. Eighteen male mice BALB/c divided into 6 groups: control group (unexposed mice); asthma group sensitized via an intraperitoneal 10µg ovalbumin/alum and exposed 1% ovalbumin; asthma followed D.metel aquoeus extract dose 0.56mg/kg BW; asthma followed D.metel aquoeus extract dose 1.12 mg/kg BW; control followed D.metel aquoeus extract dose 0.56mg/kg BW and control followed D.metel aquoeus extract dose 1.12 mg/kg BW. T cell population of CD4+ and CD8+ from spleen were quantified by BD FACSCaliburTM Flowcytometer and histopathology of bronchiolus assesssed by HE stain slides. Mice from asthma group displayed significant changes in the numbers of spleen T lymphocyte compared with control (unexposed) mice. Total T cell CD4+ increase from 12.09×106 in control, to maximum 20.09 x 106 in asthma group. Oral administration of D.metel aquoeus extract 0.56mg/kg BW, decrease significantly to T cell CD4+ population into 15.81×106, but no differences with numbers of D.metel aquoeus extract 1.12 mg/kg BW. There was significant increase in the numbers T cell CD4+ of control followed oral administration of D.metel aquoeus extract 0.56mg/kg BW increase significant than control group (untreatment of D.metel). Total T cell CD4+ increase from 12.09 in control group (untreatment with D.metel) to 20.28×106 however, there was no differences numbers of T cell CD4+ between control group and control followed D.metel aquoeus extract 1.12mg/kg BW. Total cell numbers of CD8+ significant increase from 14.6×106 in control group, to 43.03×106 in asthma group. Oral administration D.metel aquoeus extract 0.56mg/kg BW reduce T cell CD8+ in asthma group dropped to 21.05×106. However, there was no differences between the first and second doses. Oral administration D.metel to unexposed mice both first and second doses, showed there were no difference numbers T cell CD8+ than control group. Lung histology showed there was airway remodelling in asthma group after OVA challege, marked by thickening airway smooth muscle. D.metel aquoeus extract dose 0.56mg/kg BW enabled to relaxation of contraction airway  smooth muscle and returned to normal structure. However, D.metel at higher dose cause histopathology in bronchiolus, marked by accumulation of lymphocyte around bronchiolus and thickening airway smooth muscle.

Keyword: asthma, aquoeus extract, bronchiolus, D.metel, T cell lymphocyte.

27 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Apoptosis Induction in HeLa Cells by Sea Cucumber Stichopus horrens Extract

Isna Nur Fityana1, Widodo2, Muhaimin Rifa’i3

Biology Department, Mathematics and Sciences Faculty, Brawijaya University, Malang, Indonesia
nachiruka@gmail.com1, dodot134@gmail.com2, rifa123@gmail.com3

Abstract

Cancer cell is the quick and uncontrollable proliferated cells because genetic alteration. Stichopus horrens contain active compounds, which can induce apoptosis in cancer cells. This research is to understand the influence of S.horrens extract induce apoptosis in HeLa cells. We treated HeLa cells with S. horrens extract in respective doses: control (0 ug/ml), minimum dose (0,25 ug/ml), medium dose (0,50 ug/ml), and maximum dose (0,75 ug/ml). We detected the apoptosis expression with TUNEL immunocytochemistry. Amount of apoptosis cell after treatment with 0 ug/ml; 0,25 ug/ml; 0,50 ug/ml; and 0,75 ug/ml of the extract are 15,3%; 26,2%; 31,5% and 42,3%. This is show that S. horrens extract effective to be alternative for cancer therapy.

 

Keywords : apoptosis, cancer, HeLa cells, sea cucumber, Stichopus horrens.

28 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

DETECTION OF IGF-I ON DIABETES MELLITUS TYPE 2 MEDICAL PATIENT

Ratih Kusuma Wardani, Sri Widyarti, Mohammad Sasmito Djati.

Biology Department, Mathematics and Science Faculty, Brawijaya University, Malang, Indonesia

Abstract

IGF-I have biological effect like insulin IGF-I can reduce effect that cause by insulin resistant in diabetes mellitus type 2. Based on information, detection of IGF-I in DM type 2 medical patient serums is important because could become prognosis and medicine of DM type 2 medical patient. The research used ELISA method with Rabbit anti-human IGF-I (1:4000) as primer antibody and anti-rabbit IgG biotin (1:8000) as secunder antibody. Thirteen people as research subject, there are normal people and DM type 2 medical patients. Research result showed that IGF-I concentration higher than normal (34%).  The result showed that Dyslipidemia syndrome, blood high preasure, dan bronchitis cronic had not role to increase IGF-I concentration.

 

Keywords: DM type 2, IGF-I concentration

29 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Anticancer Activity of Sea Sponge Spongilla sp. Extract Induce Apoptosis in HeLa Cells

Riza Pratika (1), Widodo (1), Muhaimin Rifa’i (1)

(1)Biology Department, Mathematics and Science Faculty, Brawijaya University, Malang.
riza.icha.caq@gmail.com dodot134@gmail.com rifa123@gmail.com

Abstract

Cancer is a disease caused cells have genetic changes and changes in cell proliferation control. Spongilla sp. is known produce a bioactive anticancer. The purpose of these research is to understand the influence extract of Spongilla sp. induce apoptosis. We cultured the HeLa cells in 24 well plate using MEM as medium, then treated Spongilla sp. extract in 24 hours. We identified apoptosis using TUNEL assay. These results show that the extracts dose 1 µg/ml, 3 µg/ml, 6 µg/ml, respectively induced apoptosis 18,83%; 27,83%; 64% of cells population. Spongilla sp. induce apoptosis in HeLa cells and effective dose to induce apoptosis in HeLa cells is 6 µg / ml.

 

Keywords: Apoptosis, HeLa cells, Sea Sponge Spongilla sp.

30 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Identification of Hydrocarbon Degrading-Bacteria from Lapindo Mud Sidoarjo Based on Analysis of 16s rDNA

Purnomo, E., Ardyati, T., and Suharjono

Department of Biology, Faculty of Mathematic and Natural Science, Brawijaya University, Malang, Indonesia
tri_ardyati@yahoo.com

Abstract

Overflow of Lapindo mud in Porong, Sidoarjo has started at 2006 affected the ecological aspect around the area. One of them is the high petroleum content in the pond of Lapindo. The objectives of this study were to find potential bacteria isolates from Lapindo mud degrading-hydrocarbon (kerosene) and to identify them based on sequence of 16S rDNA. The isolation of bacteria on agar nutrient obtained 18 isolates. The screening on solid Stone Mineral Salt Solution + yeast extract (SMSSe) containing 0%, 1%, 2% and 3% of hydrocarbons shows LL3 and LL6 are able to grow on all concentration. The assay on biosurfactant production for LL3 and LL6, also LL18 as control, shows LL3 and LL6 are able to produce biosurfactant shown by micelle formation on the media, whereas LL18 does not form micelles. The tolerance of those bacteria against hydrocarbon was assay carried out by growing LL3 and LL6 on solid SMSSe with the addition of hydrocarbon concentration 0%, 5%, 10%, 15%, 25%, 35%, and 50%. The result shows LL3 is able to grow on 25% concentration of hydrocarbon, while LL6 is only able to grow on 10%. The sequencing of 16S rDNA shows LL3 is Rhodococcus sp. CH9, whereas LL6 is the Genus Staphylococcus.

 

Keywords: 16S rDNA, hydrocarbon-degrading bacteria, identification, Lapindo mud

31 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

The Potency of Antagonist Molds against Phytophthora sp. Pathogens to Apple Crops

Meisarina, N.1, Suharjono1, Otto, E.2

1Biology Department, Faculty of Mathemathic and Natural Science, Brawijaya University, Malang
meisarina1989@ymail.com
2Indonesian Citrus and Subtropical Fruits Research Institute, Batu
endarto06@yahoo.com

Abstract

The attack of Phytophthora sp. has caused the decrease of apple crops production up to 90% in Batu City. Some antagonist mold species are able to inhibit the growth of Phytophthora sp. pathogen to apple crops. The objectives of this research were to determine the characteristics of Phytophthora sp. pathogen to apple crops and to obtain antagonist mold isolates against Phytophthora. The isolation of Phytophthora sp. was conducted by baiting method and it were cultivated using medium V8 Juice Agar while antagonist molds by dilution method using Potatoes Dextrose Agar. The inhibition assay of antagonist molds against Phytophthora sp. was carried out by dual culture method. Four Phytophthora isolates (M1, M4, MB1 and MB2) were obtained as pathogen to apple crops and six molds (Trichoderma sp.1, Trichoderma sp.4, Trichoderma sp.6, Aspergillus sp.2, Aspergillus sp.3 and Penicillium sp.1) were antagonist to Phytophthora. Among the antagonist species, Penicillium sp.1 had the lowest inhibitory potency (28.82%) in contrast Trichoderma sp.6 had the highest inhibitory potency (62.95%) against the growth of Phytophthora sp.

 

Key words: Antagonist, apple, Phytophthora sp.

32 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

A model for the interaction between p53 protein and mortalin
as target protein in cancer disease

Didik H.U1., Widodo1., M. Rifa’i1.

1 Biology Departement, Faculty of Sciences, Brawijaya University, Malang, Indonesia
dknatow@gmail.com widodo@ub.ac.id rifa123@ub.ac.id

Abstract

Mortalin was found to be overexpressed in tumor cells and bind to p53 and this interaction was suggested to promote sequestration of p53 in the cytoplasm inhibit its activity . Therefore, one of strategy for cancer therapy is using small molecule to inhibit p53-mortalin interaction so p53 will promote cancer cell apoptosis. This study has aims to analyze the p53-mortalin interaction using molecular docking. The result of interaction show tetramerization domain of p53 bind to substrate binding domain of mortalin which have energy gibbs -31.89 kcal/mol and was stabilized by electrostatic interaction and hydrophobic interaction.

 

Keywords: mortalin, p53, molecular docking

33 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Comparing Carbon Stock and Soil Characters of Three Reforestation Models in Dry Low Land: Case Study of Purwodadi Botanical Garden Pasuruan

M. Yusuf1, E. Arisoesilaningsih1, G. Ekowati1, Soejono2

1Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University Malang
cupy.gathuk@gmail.com, e-arisoe@ub.ac.id, gekowati@yahoo.com
2The Indonesian Institute of Sciences-Purwodadi Botanical Garden Pasuruan
soejono@lipi.go.id

Abstract

This research aimed to compare performance of three reforestation models grown since 61 years ago for improving carbon stock and soil characters of dry low land. This research was held from August 2009 to June 2011 in three sites of reforestation area of the Purwodadi Botanical Garden. Sub vak VII C, VII E and VIII A were used as three models of reforestation. Carbon stock of each trees, poles, herb and litter in each models were recorded simultaneously with some physical and chemical soil characters. Census method was used to observe C stock of tree or poles, while sampling method was applied to measure herbs (1×1 m2) and litter (50×50 cm2) ones.The result showed that sub vak VII C and VII E  (≤ 275 Mg.ha-1) had total C stock greater than VIII A model, then trees and poles communities contributed greatest. Under different vegetation and diversity, each models showed similar soil stucture, bulk density, pH, organic matter and CEC. Soil of the models was loamy clay texture, bulk density 0.88-0.96 g.cm-3, high organic matter 4.5-7%, pH 6.0-6.5 and high CEC 34-44 me.100 g-1. Relation between biodiversity quality and soil macrofauna will be discussed in the paper.

Key words: C stock, Purwodadi Botanical Garden, reforestation models, soil characteristic

34 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

PERCEPTION OF TENGGERESE IN NGADAS TOWARD NATURE AS A IMPLEMENTATION OF JAVA PHILOSOPHY MEMAYUNG HAYUNING BAWONO

Purnomo, S.Si

Department of Biology, Faculty of Mathematics and Natural Sciences,
University Brawijaya, Malang, 2011.
omo_pug@yahoo.co.id

ABSTRACT

This study aimed to determine perception of Tenggerese in Ngadas toward nature and forms the perception. The research was conducted in the Village Ngadas, District Poncokusumo, Malang in July 2009 until March 2010. Data is collected through interviews of key persons, including chairman of the indigenous, village chiefs, community leaders and nature observations that are considered important by people of Ngadas. Data analysis was performed descriptively. Based on the survey results revealed that the community of Ngadas sacred regard to certain places both within and outside the village. Considered sacred in the village covering Pedanyangan, Sanggar Agung, Mbah Sedek Mausoleum. Meanwhile, outside the village include Punden Kutungan, Caldera Bromo, Mount Bromo, Semeru, and various other mountains in the vicinity of Bromo. In addition they also deem it land, air and water in the village is a heritage that must be maintained, and the tree is something that has a soul. Shape society’s perception of the nature that are considered sacred is the existence of rituals in these places and the effort to protect the natural riches therein. Ngadas people perception of nature and its contents is something sacred, form of this view is made sacred and the rituals shown in nature.

Key words: perception, Tenggere Ngadas, nature, Sacred place, ritual

35 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

GENETIC SIMILARITY AND HABITAT CHARACTERS OF Hermodice carunculata (PALLAS, 1766) AT COASTAL ZONES IN KABUPATEN PACITAN AND MALANG SELATAN

Hesti Kurniahu1 , Luchman Hakim2 , and Widodo3

Hesti Kurniahu,  Postgraduate Program, Brawijaya University, Malang, Indonesia.
nia_hu@yahoo.com
Luchman Hakim, Faulty Mathemathic and Sciences, Brawijaya University, Malang Indonesia
lufe_hakim@ub.ac.id
Widodo, Faculty Mathemathic and Sciences, Brawijaya Univesity, Malang Indonesia
Widodo@ub.ac.id

Abstract

Information about index similarity and habitat characters can used as conservation and domestication program. Hermodice carunculata is one of Polychaeta marine worms that live in littoral zones. The worms live at rocky or dead corals. They eat cone snails Conus sp. or dead fishes, Mollusca and Crustacea. These worms have potency as cancer drugs. The aims of this research are to know genetics population similarity and habitat characters of H. carunculata in Pacitan and Malang Selatan.

DNA isolation used Doyle and Doyle method (1987). RAPD (Random Amplified Polymorphic DNA) was applied using OPA 2 and OPA 9 as primers to amplified DNA. Amplicons analyzed using Cluster Simple Matching Coefisient analysis on MVSP 3.1 software. Abiotic factors measured directly on the fields, and its analyzed using regression analysis on Minitab 13 software. Substrates type and coordinat points of sample tracked and marked using GPS. The spatial data (characters substrates and H. carunculata distributions) analysis used many software, such as: Mapsource; AutocadMap 2004; arcView 3.3; Mapinfo; ArcGis 9.2 and Corel X3. The result showed that H. carunculata found in coral burrows at littoral zones. Mean of salinity (3,33±0,23) (3,95±0,15) ‰, pH (8,08±0,08)(8,22±0,12) and temperature (29,35±0,25)(30,72±0,72) °C. Index similarity of intra-population calculated about (0,870) higher than index similarity of inter-population (0,793). The highest index similarity of intra-population come from Ngliyep (Malang) population and the smallest from Klesem (Pacitan). While, the highest index similarity of inter-population from Balekambang and Ngliyep, the smallest index similarity of inter-population from Balekambang and Klesem. Dendogram formed three groups because location sampling distances. (Group 1: Balekambang and Ngliyep; Group 2: Godheg Kulon and Klesem; and group 3: outgroup (Perinereis cultrifera)).

 

Key words: Hermodice carunculata, Habitat Characters, RAPD, OPA 2, OPA 9.

36 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

Expression of B Lymphocyt Proliferation by Treatment Polyscias obtusa (BL) Harms with Salmonella typhi Infection Model

Noor Lianti Megasari, Muhammad Sasmito Djati, Muhaimin Rifa‘i, Widodo

Post Graduate Programs of Biology Department, Faculty of Mathematic and Science,
Brawijaya University, Indonesia
E-mail : arga_lantiza@yahoo.com

Abstract

Polyscias obtusa (BL) Harms contain phytochemicals that have the potential as imunostimulator.

Key words: Polyscias obtusa (BL) Harms, B lymphocytes, Salmonella typhi

37 International Conference on Global Resource Conservation (ICGRC 2011), Brawijaya University

REGULATORY T CELL POPULATION IN MICE INDUCTED BY Polyscias obtuse (BL.) Harms.

Sriwulan1, Moch. Sasmito Djati2, Widodo3 and Muhaimin Rifa’i4

1 Post Graduated Programe Student, Mathematic and Natural Science, Brawijaya University, Malang, Indonesia
wulanwilwatikta@gmail.com
2 Biology Lecture, Brawijaya University, malang, Indonesia
msdjati@ub.ac.id
3 Biology Lecture, Brawijaya University, Malang, Indonesia
dodot134@gmail.com
4Biology Lecture, Brawijaya University, Malang, Indonesia
rifa123@ub.ac.id

Abstract

Regulatory T cell ( CD4+CD25+) are one kind of T cell subset that play critical role to control the immune homeostasis. Some substances can promoted the population of CD4+CD25+ cell called immunoregulator. Immunoregulator substances can be natural and artificial substances. Artificial substances usually have side effect and more expensive. So that need some research to screening the natural immunoregulator that can be found from some plants. Some plants have some active compound that potent to regulate the immune condition. Those kind of plants usually used as traditional medicine, i.e. plants from genus polyscias. Polyscias obtuse (BL.) Harms as one of species from genus polyscias may contain of some active compound and also potent to develop as immunoregulator agent. The objective of this research are to investigated the population of regulator T cell in mice after treatment by P. obtuse. This research were conduct by several steps, that are preparing of P. obtuse and acclimatization the mice, gavage the mice using 4 different dose (0 mg/20 g BW; 0,5 mg/20 g BW; 2 mg/20 g BW; and 8 mg /20 g BW) for a week regularly, section and isolation the cell from spleen, doing flow cytometric analysis. Flow cytometric analysis show the percentage of cell from spleen that expressed CD4+CD25+ and the highest population of CD4+CD25+ sel are group that treat by 0,5 mg P. obtuse for 20 g BW of mice, that is 2,31%.

 

Keywords: Regulatory T cell, Polyscias obtuse

38 Asia Pacific Conference on Clinical Nutrition, Bangkok, Thailand,  5-8 Juni 2011

PENGARUH DIET SERAT GLUKOMANAN DALAM TEPUNG PORANG (Amorphophallus muelleri Blume) TERHADAP LEVEL MRNA GEN PROINSULIN PADA TIKUS DIABET

Fatchiyah

ABSTRAK

Nutrigenomik merupakan kajian studi tentang pengaruh nutrisi terhadap ekspresi genetik serta komponen genetik pada individu yang mempengaruhi metabolisme dan respon terhadap makanan. Diabetes melitus (DM) merupakan penyakit degeneratif yang salah satunya dipengaruhi oleh pola makan. DM tidak dapat disembuhkan namun dapat dikendalikan, sehingga penderita tetap dapat melakukan aktivitasnya. Diet serat sangat berperan dalam mengendalikan penyakit DM. Penelitian ini bertujuan untuk menganalisis pengaruh glukomanan dalam porang terhadap level mRNA gen proinsulin tikus diabet, sehingga dapat digunakan sebagai acuan pemanfaatan glukomanan untuk mengatasi penyakit diabet. Penelitian ini menggunakan  tikus putih (Rattus norvegicus strain wistar) jantan dengan berat badan minimal 150 g. Tikus dibagi 4 kelompok yaitu tikus non-diabet, tikus diabet, tikus diabet diberi porang 0.06 g/kg BB dan 0.12 g/kg BB. Masing-masing kelompok terdiri dari 2 ulangan. Isolasi RNA total pankreas pada semua kelompok tikus menggunakan QuickPrepTM Total RNA Extraction Kit (Amersham). Analisis mRNA gen proinsulin dilakukan melalui RNA dot blotting dengan probe cDNA. Probe cDNA dibuat dari RNA tikus non-diabet melalui RT-PCR menggunakan Ready-To-GoTM RT-PCR Beads (Amersham), dengan primer spesifik untuk gen proinsulin. cDNA hasil RT-PCR kemudian dilabel dengan biotin (Roche). Hasil penelitian menunjukkan bahwa pemberian diet serat glukomanan dalam porang dengan dosis 0.06 g/kg BB dan 0.12 g/kg BB berpengaruh terhadap peningkatan level mRNA gen proinsulin tikus diabet. Peningkatan level mRNA ini menstimulus terjadinya penurunan kadar glukosa darah pada tikus.

Kata kunci: diabet, glukomanan, mRNA gen proinsulin, porang   (Amorphophallus muelleri Blume)

39 Proceedings of The International Conference on Basic Science 17-18 Feb 2011The Study of Phosphate Released from Aquatic Sediment and Its Effect on Algal Growth

Asep Saefumillah1, Nining Betawati Prihantini2, Dini Damayanti2, Zaenab Sahamiddina1

(1)Deparment of Chemistry, Faculty of Science, Universitas Indonesia
(2)Department of Biology, Faculty of Science, Universitas Indonesia
Email: asep-s@ui.ac.id,

Abstract

Study on the release of phosphate from laboratory aquatic sediment incubation and its effect on algal growth has been conducted. The experiment shows interesting results in the context of climate change issue due to a steadly increase of global mean earth temperature on the internal loading of phosphate from sediment. Green algae Scenedesmus collected from Lake Agathis Universitas Indonesia (Prihantini et al, 2007) has been used to test the effect of phosphate from both synthetic nutrient medium and filtrate solution obtained from sedimen incubation. Firstly, Scenedesmus  is cultured in Bold Basal Medium (BBM) with the variation of high phosphate concentration (P) around the level concentration of phosphate in BBM standard, whereas other elements compositions were maintain to be constant. The second experiment was conducted with lower phosphate concentration, less than 1 ppm to approach the level phosphate concentration in the commonly actual situation in the environment. At the third experiment, different phosphate concentration used as a medium collected from sedimen incubation and synthetic medium containing different concentration of P and N. Experiment to study the release of phosphate from sedimen incubation conducted under oxic and anoxic condition, temperature and incubation time have also succesfully been conducted. The effect of of oxygen level in sedimen suspension on the phosphate release from sedimen is shown that anoxic sedimen suspension facilitates the higher phosphate release than oxic sediment suspension. The effect of temperature is very significant on the amount of phosphate release from sedimen incubation conducted under anoxic ondition. It is found  that nutrient addition in synthetic medium in contrast to control, shows the growth of algae Scenedesmus.  Experiment using filtrat sample containing different phosphate concentration from sedimen incubation produce a positive effect on the increase of algal Scenedesmus growth in line with the period of incubation time.

Keywords: phosphate sedimen, algae, eutrophication.

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