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On Saturday (25/7/2020), the Department of Biology, Universitas Brawijaya, held a community service event chaired by Drs. Sofy Permana, M.Sc., D.Sc, and consisting of Prof. Muhaimin Rifai, Ph.D., Med.Sc, Prof. Widodo, Ph.D., Med.Sc, Prof. Dr. Ir. Moch. Sasmito Djati, and was assisted by some postgraduate students of the department. This activity was titled “Deepening Immunobiodevelopment Materials for Teachers in Malang” and was attended by teachers from some senior high schools such as SMAN 3 Malang, Ar-Rahmah High School, and Sabilillah Malang High School. This event held in the midst of this pandemic run in accordance with health protocols including; provision of hand sanitizer, the use of masks, and social distancing. The community service activities included delivering material by Prof. Muhaimin Rifa’i, Ph.D., Med.Sc., observing water microorganisms, and observing lymphocyte cells using a flow cytometer.
Observation of water microorganisms was conducted using sewer water and soil samples. In this activity, participants got a demo from the committee and direct practices. Aquatic organisms were observed through a microscope so that the participants could directly observe the movement of various types of Protozoa and microalgae. In addition, participants were also invited to conduct sampling and direct observation of bacteria derived from soil samples. By using methylene blue dye, bacteria were observed under a microscope at a magnification of 1000x to determine the various forms of bacteria such as coccus and rods bacteria.
In addition to material about microorganisms, participants also got the opportunity to handle lymphocyte cells isolated from mice (Mus musculus) to be observed using a flow cytometer. In this session, participants were invited to isolate secondary lymphoid organs in the form of lymph (spleen) as observation samples. First, observations were made by comparing lymphocyte cell profiles in young and old mice. After the lymph organs were isolated, participants stained antibodies to the T lymphocyte cell population and continued with flow cytometry measurements. Through this material, it was expected that the participants had a direct picture of how the population of immune cells could be affected by the age of the individual. In addition, participants could also explain the types of lymphocyte cells based on the results of observations that have been made.
This community service was an annual agenda organized by several lecturers in the field of interest in Physiology and Cell Biology. Through this activity, participants were expected to understand the material theoretically and explain directly while practicing the results of activities as one of the practicum topics in their respective schools. Furthermore, using simple methods was expected to attract students while making it easier for students to study objects in the scope of Biology, especially those related to microorganisms and physiology.
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Pada hari Sabtu (25/7), Jurusan Biologi mengadakan acara pengabdian masyarakat yang diketuai oleh Drs. Sofy Permana, M.Sc., D.Sc, dan beranggotakan Prof. Muhaimin Rifai, Ph.D., Med.Sc, Prof. Widodo, Ph.D., Med.Sc, Prof. Dr. Ir. Moch. Sasmito Djati, dan dibantu oleh segenap mahasiswa S2 dan S3 Jurusan Biologi Universitas Brawijaya. Pengmas tersebut berjudul “Pendalaman Materi Immunobiodevelopment bagi Guru Se-Malang” dan dihadiri oleh para guru dari SMAN 3 Malang, SMA Ar-rahmah dan SMA Sabilillah Malang. Acara yang dilaksanakan di tengah pandemi ini berjalan sesuai dengan protokol kesehatan meliputi; penyediaan handsanitizer, menggunakan masker, dan bembatasan sosial. Adapun serangkaian acara Pengmas tsb antara lain penyampaian materi oleh Prof. Muhaimin Rifa’i, Ph.D., Med.Sc, praktikum pengamatan mikroorganisme air, dan praktikum pengamatan sel limfosit menggunakan flowcytometer.
Pengamatan mikroorganisme air dilakukan menggunakan sampel air selokan dan sampel tanah. Pada praktikum ini, peserta mendapat demo dari panitia dan hands on atau praktik secara langsung. Organisme air diamati melalui mikroskop sehingga dapat diamati secara langsung pergerakan berbagai jenis protozoa maupun mikroalga. Selain itu, peserta juga diajak untuk melakukan sampling dan pengamatan langsung bakteri yang berasal dari sampel tanah. Menggunakan pewarna methylen blue, bakteri diamati di bawah mikroskop pada perbesaran 1000x untuk mengetahui berbagai bentuk bakteri seperti halnya bentuk coccus dan batang.
Selain materi tentang mikroorganisme, peserta juga mendapat kesempatan handling sel limfosit yang diisolasi dari mencit (Mus musculus) untuk diamati menggunakan flowcytometer. Pada sesi ini, peserta diajak untuk isolasi organ limfoid sekunder berupa limfa (spleen) untuk dijadikan sebagai sampel pengamatan. Pengamatan dilakukan dengan membandingkan profil sel limfosit pada individu mencit muda dan tua. Setelah organ limfa diisolasi, peserta melakukan pewarnaan antibodi untuk populasi sel limfosit T dan dilanjutkan dengan pengukuran menggunakan Flow Cytometry. Melalui materi ini, diharapkan para guru yang menjadi peserta mempunyai gambaran langsung bagaimana populasi sel-sel imun dapat dipengaruhi oleh usia individu. Selain itu, para guru juga dapat menjelaskan jenis-jenis sel limfosit berdasarkan hasil pengamatan yang telah dilakukan.
Pengabdian masyarakat ini merupakan agenda tahunan yang diselenggarakan oleh beberapa dosen dalam bidang minat Fisiologi dan Biologi Sel. Melalui kegiatan ini diharapkan para guru tidak hanya menguasai materi secara teoritis, namun dapat menjelaskan secara langsung sekaligus mampu mempraktikkan hasil kegiatan sebagai salah satu topik praktikum di sekolah masing-masing. Dengan penggunaan metode yang sederhana, diharapkan dapat menarik minat siswa sekaligus memudahkan siswa dalam mempelajari obyek-obyek kajian dalam ruang lingkup Biologi, terutama yang berkaitan dengan mikroorganisme dan fisiologi.
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